n a cd4 pe cy7 canine ykix302 9 ebioscience 25  (Thermo Fisher)

Journal: Developmental and comparative immunology

Article Title: Multi-color Flow Cytometry for Evaluating Age-Related Changes in Memory Lymphocyte Subsets in Dogs

doi: 10.1016/j.dci.2018.05.022

Figure Lengend Snippet: Cell surface and intracellular antibodies and markers used in flow cytometry studies

Article Snippet: For intracellular cytokine staining experiments, both mitogen-stimulated and control cells were concurrently incubated with Brefeldin A (B7651, Sigma-Aldrich, Saint Louis, MO, USA) at a dilution of 1 μl/ml in complete media during the 6-hour stimulation period. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antigen/ marker Conjugated fluorochrome Antigen species Antibody clone # Manufacturer Catalogue # Dilution Reference Cell Surface Viability dye Near infra-red N/A N/A Thermo Fisher {"type":"entrez-nucleotide","attrs":{"text":"L34975","term_id":"522218","term_text":"L34975"}} L34975 1:1000 N/A CD4 PE-Cy7 Canine YKIX302.9 eBioscience 25-5040-41 1:10 N/A CD4 PB Canine YKIX302.9 Biorad MCA1038PB 1:10 N/A CD8 PerCp-Cy5.5 Canine YCATE55.9 eBioscience 46-5080-41 1:25 N/A CD28 APC Canine 5B8 eBioscience 17-0282-41 1:10 N/A CD44 FITC Canine YKIX337.8 Thermo Fisher 11-5440-41 1:10 N/A CD62L PE Human FMC46 BioRad MCA1076PET 1:10 ( Bismarck et al., 2012 ; Hartley and Tarleton, 2015 ; Rothe et al., 2017 ; Schuberth et al., 2007 ) CD45RA Biotin Canine CA4.1D3 PFM Lab N/A 1:50 ( Cobbold and Metcalfe, 1994 ; Reis et al., 2005 ) Streptavadin BV711 N/A N/A Biolegend 405241 1:50 N/A CD25 PE Canine P4A10 eBioscience 12-0250-42 1:10 N/A Intracellular CD3 PB Human CD3-12 BioRad MCA1477PB 1:50 ( Monjazeb et al., 2016 ) CD3 FITC Human CD3-12 BioRad MCA1477F 1:50 ( Monjazeb et al., 2016 ) IFNγ PE Bovine CC302 BioRad MCA1783PE 1:10 ( Hartley and Tarleton, 2015 ; Pedersen et al., 2002 ) TNFα BV-785 Human MAB-11 Biolegend 502947 1:25 ( Moreira et al., 2015 ) Ki67 PE-Cy7 Human 20Raj1 eBioscience 25-5699-42 1:50 ( Galkowska et al., 1996 ) Open in a separate window APC, allophycocyanin; BV-711, brilliant violet™711; BV-785, brilliant violet™785; FITC, fluorescein; PB, pacific blue; PE, phycoerythrin; PE-Cy7, PE-cyanine 7; PE-TR, PE-texas red; PerCPCy5.5, peridinin chlorphyll protein-cyanine 5.5; PFM lab, Peter F. Moore laboratory; N/A, not applicable.

Techniques: Flow Cytometry, Marker